Autopatching: Interneurons, Synapses, and Dendrites
In vivo patch-clamp is the gold standard for intracellular recordings, but it is a very manual and highly skilled technique. We have created the most automated in vivo patch-clamp robot to date, by enabling production of multiple, serial intracellular recordings without human intervention. Our robot automates pipette filling, Ag/AgCl wire threading, pipette positioning, neuron hunting, break-in, delivering sensory stimulus, and recording quality control, enabling in vivo cell-type characterization.
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M.C. Yip, C.F. Lewallen, C.R. Landry, I. Kolb, W.A. Stoy, C.R. Forest, Patch-walking: Coordinated multi-pipette patch clamping to efficiently discover synaptic connections, Proceedings of the Annual Meeting of the Society for Neuroscience (SfN), Chicago, IL, October 19-23 2019.
G.L. Holst, W. Stoy, B. Yang, I. Kolb, S.B. Kodandaramaiah, L. Li, U. Knoblich, H. Zeng, B. Haider, E.S. Boyden, C.R. Forest, Autonomous patch clamp robot for functional characterization of neurons in vivo: development and application to mouse visual cortex, J. Neurophysiology 121:2341- 2357, 2019. https://doi.org/10.1152/jn.00738.2018[PDF]
I. Kolb, G.T. Franzesi, M. Wang, S.B. Kodandaramaiah, C.R. Forest, E.S. Boyden, A.C. Singer, Evidence for long-timescale patterns of synaptic inputs in CA1 of awake behaving mice, Journal of Neuroscience 26 December 2017, 1519-17; DOI: https://doi.org/10.1523/JNEUROSCI.1519-17.2017[PDF]
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W.A. Stoy, I. Kolb, G.L. Holst, Y. Liew, A. Pala, B. Yang, S.B. Kodandaramaiah, E.S. Boyden, G.B. Stanley, C.R. Forest, Robotic navigation to sub-cortical neural tissue for single cell electrophysiology in vivo, J. Neurophysiology, 7 June 2017, doi: 10.1152/jn.00117.2017[PDF]
H.-J. Suk, I. vanWelie, S.B. Kodandaramaiah, B. Allen, C.R. Forest, E.S. Boyden, Fully automated, closed-loop, two-photon image-guided patch clamp neural recording in vivo, Proceedings of the Annual Meeting of the Society for Neuroscience (Neuroscience 2016), San Diego, CA, Nov 12-16, 2016.
S.B. Kodandaramaiah, G.B. Holst, I.R. Wickersham, A.C. Singer, G.T. Franzesi, M. McKinnon, C.R. Forest, E.S. Boyden, Assembly and operation of an autopatcher: a robot for automated intracellular neural recording in vivo, Nature Protocols, 11, 634-654 (2016). doi:10.1038/nprot.2016.007
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