Genetically-Encoded Voltage Indicators and Effectors
We are extending on our prior pioneering work on automated patch clamping robots by developing a robotic patch clamp system for optogenetic tool screening. We will then apply this technology to improve the kinetics of different optogenetic molecules, and also seek to improve the performance of an important class of optogenetic tool – improving the selectivity and conductances of light-gated potassium channels.
P.Y. Borden, A.D. Ortiz, C. Waiblinger, A.J. Sederberg, A.E. Morrissette, C.R. Forest, D. Jaeger, G.B. Stanley, Genetically expressed voltage sensor ArcLight for imaging large scale cortical activity in the anesthetized and awake Mouse, Neurophotonics, 4(3), 031212 (2017), doi: 10.1117/1.NPh.4.3.031212[PDF]
H.H. Wang, F.J. Isaacs, P.A. Carr, Z.Z. Sun, G. Xu, C.R. Forest, G.M. Church, Programming cells by multiplex genome engineering and accelerated evolution, Nature, Vol. 460, p. 894-898, July 2009.[PDF]
C.R. Forest, B. Woodruff, D. Buckley, W.G. Thilly, and I.W. Hunter, Assembly and Constraint Technology for Large Arrays of Capillaries, Precision Engineering, Vol 33(3), p. 275-283, July 2007.[PDF]