Whole-cell patch clamp electrophysiology of neurons, although a gold standard technique for high-fidelity analysis of the biophysical mechanisms of neural computation and pathology, requires great skill to perform. We have developed a simple robot that automatically performs patch clamping in vivo, algorithmically detecting cells by analyzing the temporal sequence of electrode impedance changes. We demonstrate good yield, throughput, and quality of recording in mouse cortex and hippocampus.
Ed Boyden (MIT)
- S. Kodandaramaiah, G. Franzesi, B. Chow, E. Boyden, C.R. Forest, Automated whole-cell patch clamp electrophysiology of neurons in vivo. Nature Methods. (http://dx.doi.org/10.1038/
NMETH.1993). [.pdf] [supplemental methods] [supplemental software]
- M.V. Baratta, S.B. Kodandaramaiah, P.E. Monahan, P.-A. Lin, K. Kim, A. Yang, C.R. Forest, K.A. Goosens, E.S. Boyden, Temporally precise contribution of serotonergic neural activity to stress-induced enhancement of fear learning, (submitted)
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Interested in your own autopatcher robot?
Visit http://autopatcher.org/ for a parts list, manual, and software to make your own autopatcher robot.
Visit http://neuromaticdevices.com/ to purchase ready-to-use custom installations and training.